Quantitative Measurement of Eotaxin and RANTES mRNA in the Nasal Polyps.
- Author:
Chang Ho JEON
1
;
Hun Suk SUH
;
Seung Hun SHIN
Author Information
1. Department of Clinical Pathology, College of Medicine, Catholic University of Taegu Hyosung, Taegu, Korea.
- Publication Type:Original Article
- Keywords:
Nasal polyp;
Eotaxin;
RANTES;
Eosinophils and Quantitative PCR
- MeSH:
Chemokine CCL5*;
Daegu;
DNA;
Eosinophilia;
Eosinophils;
Humans;
Nasal Polyps*;
Polyps;
RNA, Messenger*;
Tuberculosis;
Turbinates
- From:Korean Journal of Clinical Pathology
2000;20(4):424-429
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Nasal polyps were developed from eosinophil infiltrations and activation by chronic inflammatory reactions. Eotaxin and RANTES have been postulated to be involved in the recruitment and activation of eosinophils to the inflamed tissues. The aim of this study is to estimate the mRNA expression of eotaxin and RNATES in the nasal polyps and it's effect on tissue eosinophils. METHODS: At first, we evaluated the linearity and precision of GeneAmp 5700R(PE Applied Biosystems, Foster, U.S.A) with M. tuberculosis DNA. We collected 17 allergic, 30 non-allergic nasal polyps and 15 normal inferior turbinates from the patients visiting Catholic University Hospital of Taegu Hyosung. We performed the quantitative polymerase chain reaction(PCR) for the eotaxin and RNATES, and the tissue immunohistochemical stain for the major basic protein. RESULTS: GeneAmp 5700R disclosed good linearity and precision. Compared with the normal inferior turbinates, eotaxin mRNA levels were increased in the allergic and non-allergic polyps, and showed significant correlation with eosinophils infiltration and activation. But the RANTES didn't revealed any significant differences among these groups, and no correlation with tissue eosinophils. The patients with allergic polyps showed increased eosinophils infiltration and activation in the tissue, while those with non allergic polyps disclosed increased eosinophils activation. CONCLUSIONS: Since eotaxin expression were increased in the tissue of the patients with nasal polyps and showed good correlation with eosinophils infiltration and activation in the tissue, it had been considered that eotaxin played an important role in the pathogenesis of allergic polyps and tissue eosinophilia.
