Transcriptional activities of tumor-specific survivin promoter and PSMA promoter and enhancer in human prostate cancer: evaluation and comparison.
- Author:
Xiao-Mei LUO
1
;
Jia-Yun LIU
;
Ming-Quan SU
;
Xiao-Ke HAO
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, Surface; genetics; Cell Line, Tumor; Glutamate Carboxypeptidase II; genetics; Humans; Inhibitor of Apoptosis Proteins; genetics; Male; Plasmids; Promoter Regions, Genetic; Prostatic Neoplasms; genetics; therapy; Transcription Initiation Site; Transcriptional Activation; Transfection
- From: National Journal of Andrology 2012;18(2):122-125
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect and compare the transcriptional activities of prostate-specific membrane antigen (PSMA) promoter and enhancer and survivin promoter in different human prostate cancer cell lines, and to search for some evidence for the targeting gene therapy of human prostate cancer.
METHODSThe fragments of the PSMA promoter and enhancer and survivin promoter were amplified by PCR and inserted into pGL3-Basic. The recombinant plasmids were transiently transfected into human prostate cancer cell lines and normal Chang liver cells, and, their transcriptional activities in various cells were determined by measuring the expression of luciferase.
RESULTSThe survivin promoter exhibited a higher transcriptional activity than PSMA promoter and enhancer in tumor cell lines, and the S2pro promoter showed the highest activity, reaching one third of that of the CMV promoter.
CONCLUSIONThe survivin promoter is highly activated in prostate cancer cell lines and may serve as a new tool for the transcriptional targeting gene therapy of prostate cancer.
