Decreased mRNA expressions of T-type channel alpha1H and alpha1G in the sperm of varicocele patients and their implication.
- Author:
Shi-Ping WANG
1
;
Pei-Tao WANG
;
Feng GAO
;
Qiang LI
;
Zhong-Qiang LIU
;
Xiu-Yan FU
;
Hua-Qin LI
;
Xin-Sheng WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Calcium Channels, T-Type; genetics; metabolism; Case-Control Studies; Humans; Infertility, Male; genetics; Male; RNA, Messenger; genetics; Semen Analysis; Spermatozoa; metabolism; Varicocele; genetics; metabolism; Young Adult
- From: National Journal of Andrology 2012;18(4):331-334
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the relationship of varicocele (VC) with the expressions of T-type channel alpha1H and alpha1G in the sperm of VC patients.
METHODSBased on the WHO criteria, we examined the semen samples by computer-aided sperm analysis (CASA), and divided the samples into groups A (normal semen from volunteers, n = 20), B (normal semen from VC patients, n = 16) and C (abnormal semen from VC patients, n = 44). We optimized the semen by discontinuous Percoll grade centrifugation, and determined the mRNA expressions of T-type channel alpha1H and alpha1G in the three groups using using reverse transcription polymerase chain reaction (RT-PCR).
RESULTSCompared with group A, the mRNA expressions of alpha1H and alpha1G showed with no significant decrease in group B (P>0.05), but were remarkably reduced in group C (P<0.01).
CONCLUSIONThe abnormal mRNA expressions of T-type channel alpha1H and alpha1G may be one of the causes of declined semen quality and consequently infertility in VC patients, which has pointed out a new direction for the studies of the causes and treatment of VC-related infertility.
