Human osteoblasts response to different magnitudes of mechanical stimulation in vitro.
- Author:
Jun-feng ZHU
1
;
Xian-long ZHANG
;
Cheng-tao WANG
;
Xiao-ling ZHANG
;
Xiao-chun PENG
;
Xue-ling BAI
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Cell Line; Cell Proliferation; Collagen Type I; metabolism; Core Binding Factor Alpha 1 Subunit; metabolism; Gene Expression Regulation; Humans; Osteoblasts; cytology; metabolism; Osteocalcin; metabolism; Stress, Mechanical
- From: Chinese Journal of Surgery 2009;47(5):381-384
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of different magnitudes of tensile strain on human osteoblasts differentiation.
METHODSAccording to the strain amplification mechanism at cellular level and a data calculated by finite element analysis, the cellular level strain of 0.8%, 1.6%, 2.4% and 3.2% was respectively applied to human osteoblasts for 48 h at a frequency of 1 Hz. Alkaline phosphatase activity and the expression of osteoblast-related genes were detected by Semi-Quantitative RT-PCR and densitometric analysis.
RESULTSAlkaline phosphatase activity significantly increased at 0.8% and 1.6%. The level of osteocalcin mRNA increased at 2.4% and 3.2%. Cbfa1/Runx2 gene expression only increased at 3.2%. Comparing to static control, mRNA level of type I collagen increased at every magnitude. The mRNA level decreased at 0.8% and increased at 3.2% when compared to the group with 1.6% elongation.
CONCLUSIONSHigher magnitudes of strain enhance expression of osteocalcin, type I collagen gene and Cbfa1/Runx2 in human osteoblasts, but lost the ability to increase ALP activity which is remained by lower magnitudes of strain. Type I collagen gene expression increases in a strain magnitude dependent manner.
