Association of microRNA-related genes (DROSHA, DICER1 and GEMIN4) polymorphisms with T-cell lymphoma prognosis.

Xiaobo Tian; Bo Zhang; Xi LI; Kan Zhai; Jian XU; Jiang Chang; Yan Qiao; Yuling Zhou; Liming Huang; Jieping Chen

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Association of microRNA-related genes (DROSHA, DICER1 and GEMIN4) polymorphisms with T-cell lymphoma prognosis.

Author: Xiaobo TIAN ¹ ; Bo ZHANG ¹ ; Xi LI ¹ ; Kan ZHAI ¹ ; Jian XU ¹ ; Jiang CHANG ¹ ; Yan QIAO ¹ ; Yuling ZHOU ¹ ; Liming HUANG ¹ ; Jieping CHEN ¹
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1. Department of Hematology, Southwest Hospital, Third Military Medical University, Chongqing 400038, China.
Publication Type:Journal Article
MeSH: DEAD-box RNA Helicases; genetics; Genetic Predisposition to Disease; Genotype; Humans; Lymphoma, T-Cell; diagnosis; genetics; MicroRNAs; genetics; Minor Histocompatibility Antigens; Polymerase Chain Reaction; Polymorphism, Single Nucleotide; Prognosis; Ribonuclease III; genetics; Ribonucleoproteins, Small Nuclear; genetics
From: Chinese Journal of Hematology 2014;35(5):408-413
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo analyze the association of micoRNA-related genes DROSHA single nucleotide polymorphisms (SNP) rs10719 and rs6877842, DICER1 rs3742330and GEMIN4 rs3744741 with prognosis of T-cell lymphoma.
METHODSPolymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method was used to determine the genotypes of the above 4SNPs and their associations with complete remission (CR) rate and overall survival (OS) in 163 patients with TCL.
RESULTSPatients carrying the rs6877842 CG genotype had a significantly higher CR rate compared with those carrying the CC genotype (OR=0.07, 95% CI 0.01-0.72, P=0.026); the same for patients carrying the DICER1 rs3742330 GG genotype compared with those carrying the GA genotype (OR=0.15, 95% CI 0.02-0.97, P=0.047) or the AA genotype (OR=0.11, 95% CI 0.02-0.71, P=0.020). In addition, patients with the DICER1 rs3742330 GG genotype had a significantly improved OS compared with those carrying the GA (HR=9.02, 95% CI 1.22-66.92, P=0.031) or AA genotype (HR=8.77, 95% CI 1.19-64.67, P=0.033). The other two SNPs of rs10719 and rs3744741 had no significant association with CR or OS.
CONCLUSIONDROSHA rs6877842 and DICER1 rs3742330 were independent factors for TCL CR, and DICER1 rs3742330 was also an independent prognostic factor for TCL OS.