Application of three-probe fluorescence in situ hybridization panel in the diagnosis of pediatric B cell acute lymphoblastic leukemia.
- Author:
Jing FAN
1
;
Chengwen LI
1
;
Jiawei ZHAO
1
;
Jinying GONG
1
;
Yingchun ZHENG
1
;
Kun RU
1
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Child; Child, Preschool; Female; Humans; In Situ Hybridization, Fluorescence; methods; Infant; Karyotyping; Male; Precursor Cell Lymphoblastic Leukemia-Lymphoma; diagnosis; genetics
- From: Chinese Journal of Hematology 2014;35(6):542-545
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the three-probe fluorescence in situ hybridization (FISH) panel in the diagnosis of pediatric B cell acute lymphoblastic leukemia (B-ALL).
METHODSThree-probe (TELAML1, BCR-ABL and MLL) FISH and conventional cytogenetic analysis were performed in 207 children with B-ALL.
RESULTSIn 207 B-ALL children, the three-probe FISH panel assay showed that 151 cases carried genetic aberrancies with a positive rate of 72.9%, including 44 cases with typical positive signal patterns and 148 cases with atypical signal patterns (among them 41 cases have multiprobe abberancy). The conventional cytogenetic analysis detected 53 cases chromosomal abnormality with a positive rate of 25.6%.
CONCLUSIONThe detection rate of genetic abnormalities in newly- diagnosed pediatric B-ALL could be significantly improved by using three-probe FISH panel upon conventional cytogenetic analysis.
