The Role of IκBα in TNF-α-induced Apoptosis in Hepatic Stellate Cell Line HSC-T6
- Author:
Zhijun QU
1
;
Duande LOU
;
Yanfeng PAN
Author Information
1. Longgang Central District Hospital
- Keywords:
nuclear factor-κB;
nuclear factor inhibiting protein;
tumor necrosis factor-α;
hepatic stellate cells;
apoptosis
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(4):407-410
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the role of NF-κB in TNF-α induced apoptosis in HSC-T6, a mutant IκBα was transfected into HSC-T6 cells by lipofectin transfection technique and its transient effect was examined 48 h after the transfection. The activation of NF-κB was detected by immune fluorescence cytochemistry and Western blotting with anti-p65 antibody. The apoptosis and the rate of inhibition by TNF-α in both transfected and untransfected HSC-T6 cells were measured respectively by FAC-Scan side scatter analysis and MTT methods. Our results showed that TNF-α could activate NF-κB in untransfected cells but not in transfected HSC-T6 cells. The percentage of apoptosis in transfected cells were significantly higher than that in the untransfected ones (P＜0.01) and it was also true of the inhibition rate (P＜0.01). It is concluded that the resistance of HSC-T6 towards apoptosis induced by TNF-α can be mediated by NF-κB activation. The inhibition of NF-κB activation by mutant Iκ Bα can attenuate the resistance of HSC-T6 cells and increase its sensitivity to TNF-α.
