The Expression of Cyclooxygenase-2 in Cervical Cancers and Hela Cells Was Regulated by Estrogen/Progestogen
- Author:
Yunguang LI
1
;
Demin PU
;
Yanli LI
Author Information
1. 山东大学附属齐鲁医院
- Keywords:
cervical cancer;
cyclooxygenase-2;
menstrual cycle;
17-β-estradiol;
medroxyprogesterone acetate
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(4):457-460
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the relationship between the expression of cyclooxygenase-2 (COX-2) and menstrual cycle, the regulatory effects of 17-β-estradiol (E2) and medroxyprogesterone acetate (MPA) on the expression of COX-2 in cervical cancer Hela cells were examined. Cervical cancer specimens were obtained from 47 pre-menopausal patients. The phase of menstrual cycle was determined by case history and HE staining of uterine endometrium. COX-2 was immunohistochemically stained by SABC staining and the staining intensity was determined with computerized image analysis system.Hela cells were incubated with alcohol, E2, E2+MPA, MPA for 12, 24 and 48 h respectively. The expression of COX-2 in Hela cells was detected by Western blotting and reverse transcriptase-polymerase chain reaction (RT-PCR). Our results showed that the expression of COX-2 was significantly higher during proliferative phase than secretory phase (P＜0.05), but there was no difference in the positive rate between proliferative phase and secretory phase (P＞0.05). Incubation with E2 could significantly enhance the expression of COX-2 continually. On the contrary, E2+MPA and MPA alone could decrease the expression of COX-2 as compared with the control and E2 group (P＜0.05 and P＜0.01 respectively). It is concluded that the expression of COX-2 in cervical cancer of pre-menopausal patients and Hela cells was regulated by estrogen/progestogen.
