Effect of Stathmin Decoy-oligodeoxynucleotides on the Proliferation and Differentiation of Precartilainous Stem Cells
- Author:
Fengjing GUO
1
;
Yibei ZHANG
;
Anmin CHEN
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
decoy-oligodeoxynucleotides;
Stathmin gene;
precartilainous stem cells;
proliferation;
differentiation
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(5):557-560
- CountryChina
- Language:Chinese
-
Abstract:
By using decoy-oligodeoxynucleotides (decoy-ODNS) technique, the effects of Stathmin gene on the proliferation and differentiation of in vitro cultured precartilainous stem cells (PSCs) were investigated. The Stathmin decoy-ODNs were transfected into PSCs in rats by using gene trans- fection technique. Under the induction of cortisol (1 μmol/L), electrophoretic mobility shift assay was used the inhibitory effects of decoy-ODNS on Stathmin gene. MTT and cytometry were used to test the cell proliferation. The expression of collagen Ⅱ and Ⅴ and Stathmin protein was detected by using Western blot. The results showed that Stathmin decoy-ODNs inhibited the Stathmin activity in a dose-dependent manner. When the concentration of decoy-ODNs was 10 times of standard con- centration, the proliferation of PSCs was obviously suppressed and the differentiation happened. Compared to the control group, the difference was significant (P<0.05). It was concluded that de-coy-ODNs could inhibit the proliferation and promote the differentiation of PSCs by antagonizing Stathmin activity.
