siRNA Specific to Pdx-1 Disturbed the Formation of the Islet in Early Zebrafish Embryos
- Author:
Shen CHEN
1
;
Jintao HUANG
;
Guangnfing YUAN
;
Qian CHEN
;
Nannan HUANG
;
Fukang XIE
Author Information
1. 中山大学
- Keywords:
Pancreas;
Pancreatic development;
pdx-1;
siRNA;
Zebrafish
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(6):639-642
- CountryChina
- Language:Chinese
-
Abstract:
Pdx-1, an important transcription factor highlighting in the early pancreatic development,islet functions and pancreatic disorders, needs to be more investigated in zebrafish, and siRNA is still seldom applied in zebrafish embryo-related research.Our aim was to explore the role of pdx-1 in pan-creatic development of zebrafish embryos by using siRNA approach. Microinjection, reverse tran-scriptase-PCR (RT-PCR), in situ hybridization and immunofluorescent staining were used in this re-search, and the morphology of the islet in normal zebrafish embryos, and in those treated with the siRNA specific to pdx-1 (siPDX-1) or siGFP was observed and compared. The expression of pdx-1 was detected in the stages of 1-cell, 2-cell, 4-cell, 8-cell, 16-cell, 16-hour by RT-PCT. The in situ hy-bridization and immunofluorescent staining results showed that siPDX-I disturbed the formation of the islet in zebrafish embryos. Pdx-1 played multiple roles in maintaining the phenotype of the islet during embryogenesis in zebrafish.
