Protective Effect of Paeoniflorin against Optic Nerve Crush
- Author:
Jing LI
1
;
Xinchun XIONG
;
Yunhai LIU
Author Information
1. 华中科技大学同济医学院附属同济医院
- Keywords:
paeoniflorin;
optic nerve crush;
retina ganglion cell;
retrograde labeling
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2007;27(6):650-652
- CountryChina
- Language:Chinese
-
Abstract:
In order to evaluate the efficacy of traditional paeonia extract paeoniflorin against optic nerve crush, 16 Brown Norway rats were divided into two groups at random, with 8 rats in each group. In paeoniaflorin-treated group, 2 mg paeoniaflorin (total volum: 1 mL) was injected into rat's peritoneum one time a day for a period of 8 days. Rats in untreated group were given a single dose of vehicle. The optic nerve was crushed by a special forceps for 30 s in the left eye and a sham proce-dure was performed in the right eye on the 2nd day after the first injection. The retrograde fluorogold labeling of ganglion cells was conducted 5 days after optic nerve crush. The whole retina was flat-mounted thereafter. The ganglion cells that survived the crush were counted under fluorescent microscope by using an automatic counting software. As compared with the contralateral eye, the survival rate of ganglion cells in the left eye increased from 40.22% to 64.53% with a significant dif- ference found between them (t=2.55, P=0.023). The results suggested that the paeonia extract paeoniflorin possessed a protective effect against optic nerve crush.
