Determination of taxifolin in Polygonum orientale of different storage period.
- Author:
Ya-Nan TAN
1
;
Miao-Miao TONG
2
;
Yu-Yao ZHANG
2
;
Yang-Yang XU
2
;
Yan-Jun ZHAI
2
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Drug Stability; Drug Storage; methods; Drugs, Chinese Herbal; analysis; Polygonum; chemistry; Quercetin; analogs & derivatives; analysis
- From: China Journal of Chinese Materia Medica 2013;38(17):2779-2781
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo find out the correlation between the content of taxifolin in Polygonum orientale and the storage time.
METHODHPLC was used to determine taxifolin. The chromatographic condition was as following: Diamonsil C18 column (4.6 mm x 200 mm, 5 microm), mobile phase acetonitrile -0.1% phosphoric acid (gradient elution), the detection wavelength 290 nm and flow rate 1.0 mL x min(-1), the column temperature 30 degrees C.
RESULTThe injection volume of taxifolin was in good linearity within 0.07 and 0.35 microg, the average recovery was 99.7% with RSD 0.2%. Taxifolin content was 0.84, 1.36, 1.75, 1.99 mg x g(-1) corresponding to storage time of 10, 7, 6, 5 years, respectively.
CONCLUSIONThe content of taxifolin decreased with the storage time. When the storage period is more than six years, the content is lower than that required by Chinese Pharmacopoeia (2010 version). This method has a good repeatability and accuracy, it provides a scientific reference for clinical use and quality evaluation of P. orientale.
