Determination of eight ginsenosides in renshenshouwu capsules by HPLC-DAD.
- Author:
Li-Na JI
1
;
Wei-Hong FENG
2
;
Zhi-Min WANG
2
;
Jing-Jing ZHU
2
;
Qi-Wei ZHANG
2
;
Liang-Mian CHEN
2
;
Chun LI
2
Author Information
- Publication Type:Journal Article
- MeSH: Capsules; analysis; Chromatography, High Pressure Liquid; instrumentation; methods; Drugs, Chinese Herbal; analysis; Ginsenosides; analysis
- From: China Journal of Chinese Materia Medica 2013;38(17):2798-2802
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an HPLC-DAD method for simultaneous determination of eight ginsenosides in Renshenshouwu capsules.
METHODUltimate C18 column (4.6 mm x 250 mm, 5 microm) was adopted for gradient elution, with acetonitrile and water as the mobile phase. The flow rate was 1 mL x min(-1), the column temperature was set at 30 degrees C, and the detection wavelength was 203 nm.
RESULTA good linearity was observed in the range of 0.242-12.1, 0.222-11.1, 0.251-25.1, 0. 245-24.5, 0.232-23.2, 0.232-23.2, 0.264-26.4, 0.244-24.4 microg for ginsenoside Rg1, Re, Rb1, Rc, Rb2, Rb3, Rd and 20(S)-ginsenoside Rg3, respectively, with the average recoveries of 102.7%, 103.2%, 101.6%, 101.2%, 102.0%, 100.7%, 101.9%, 102.0%, respectively.
CONCLUSIONThe method was so simple, accurate and effective that it could be used for quality control of the above eight components in Renshenshouwu capsules.
