Application of solid-phase extraction column for determination of matrine and oxymatrine in Sophora flavescens.
- Author:
Xia YANG
1
;
Bao-Lin GUO
2
;
Hong-Yu HU
3
;
Wen-Hua HUANG
2
;
He-Ping QIAO
4
;
Sheng-Ci FAN
4
;
Zha-Gen GUAN
4
Author Information
1. Shanxi Zhengdong Pharmaceutical Co., Ltd., Changzhi 047100, China. yangxiaxy@yeah.net
2. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical college, Beijing 100193, China.
3. Beijing Zhengdong Guangming Pharmaceutical Research Co., Ltd., Beijing 100120, China.
4. Shanxi Zhengdong Pharmaceutical Co., Ltd., Changzhi 047100, China.
- Publication Type:Journal Article
- MeSH:
Alkaloids;
analysis;
isolation & purification;
Chromatography, High Pressure Liquid;
methods;
Drugs, Chinese Herbal;
analysis;
isolation & purification;
Quinolizines;
analysis;
isolation & purification;
Solid Phase Extraction;
methods;
Sophora;
chemistry
- From:
China Journal of Chinese Materia Medica
2013;38(17):2844-2847
- CountryChina
- Language:Chinese
-
Abstract:
A Cleanert Alumina-N-SPE column (0.5 g/6 mL) chromatograpy with 5 mL of chloroform-methanol (7: 3) as eluent, instead of aluminum oxide column (100-200 mesh, 5 g, 1 cm) chromatograpy eluted successively with chloroform and the chloroform-methanol (7:3) (20 mL each), was applied to enrich matrine and oxymatrine in Sophora flavescens. Also, the optimization of the HPLC determination conditions with acetonitrile-ethanol absolute-3% phosphoric acid solution (84: 6: 10) as mobile phase, instead of acetonitrile-ethanol absolute -3% Phosphoric acid solution (80: 10: 10) recorded in Chinese Pharmacopoeia 2010 Edition, was more suitable for determination of matrine and oxymatrine in S. flavescens. This method has advantage of reducing sample handling time and solvent volume and increasing the accuracy and feasibility, which can simplify the procedure for determination of matrine and oxymatrine in S. flavescens.
