Study on anti-aging effect of ginsenoside Rg1 in serial transplantation of hematopoietic stem cells and progenitor cells.

Yue Zhou; Jian-wei Wang; Rong Jiang; Xin Yao; Bing Yang; Shi-zhong Cai; Jun Liu; Dian-feng Liu; Ya-ping Wang

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Study on anti-aging effect of ginsenoside Rg1 in serial transplantation of hematopoietic stem cells and progenitor cells.

Author: Yue ZHOU ¹ ; Jian-Wei WANG ² ; Rong JIANG ² ; Xin YAO ² ; Bing YANG ² ; Shi-Zhong CAI ² ; Jun LIU ² ; Dian-Feng LIU ² ; Ya-Ping WANG ²
Author Information

1. Engineering Research Room of Stem Cells and Tissues, Teaching and Research Room of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China. zhouyue_120@sina.com
2. Engineering Research Room of Stem Cells and Tissues, Teaching and Research Room of Histology and Embryology, Chongqing Medical University, Chongqing 400016, China.
Publication Type:Journal Article
MeSH: Aging; drug effects; metabolism; Animals; Antigens, Ly; genetics; metabolism; Cell Cycle; drug effects; Female; Ginsenosides; pharmacology; Hematopoietic Stem Cell Transplantation; Hematopoietic Stem Cells; cytology; drug effects; metabolism; Humans; Male; Membrane Proteins; genetics; metabolism; Mice; Mice, Inbred C57BL
From: China Journal of Chinese Materia Medica 2013;38(17):2848-2853
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the anti-aging effect of ginsenoside R1 in serial transplantation of hematopoietic stem cells and progenitor cells.
METHODHSC/HPC aging model in vivo was established through the Sca-1 (+) HSC/HPC serial transplantation of male donor mice that had been separated and purified by the magnetic-activated cell sorting method. The female recipient mice that had been radiated with lethal dose of 60Co gamma ray were divided into four groups: the control group, the aging group, the Rg1-treated aging group and the Rg1 anti-aging group. The expression of Sry genes in bone marrow cells of recipient mice was analyzed by fluorescence quantitative PCR, in order to determine the source of hematopoietic reconstruction cells, observe the survival time and the recovery of the hematology of peripheral blood, and study the reconstruction of the hematopoietic function of recipient mice, the hematopoietic recovery promoted by Rg1, the culture of CFU-Mix of hemopoietic progenitor cells, the cell cycle analysis and aging-related SA-beta-Gal staining analysis on biological characteristics of Sca-1 (+) HSC/HPC aging, and the effect of Rg1 in vivo regulation on Sca-1 + HSC/HPC aging.
RESULTThe hematopoietic reconstruction cells of female recipient mice were derived from male donor mice. With the serial transplantation, the 30-day survival rate and the hematology in peripheral blood of recipient mice decreased. Sca-1 (+) HSC/HPC showed aging characteristics: the ratio of cells in G0/G1 phase and the positive rate of SA-beta-gal staining increased, whereas the number of CFU-Mix decreased. Compared with the aging group of the same generation, Rg1 -treated aging group and Rg1 anti-aging group showed higher 30-day survival rate and WBC, HCT, PLT and CFU-Mix, and lower cell ratio in Sca-1 (+) HSC/HPC G0/G1 stage and positive rate of SA-beta-gal staining. The Rg1 anti-aging group showed more significant changes than the Rg1 -treated aging group.
CONCLUSIONGinsenoside Rg1 has the effect of delaying and treating Sca-1 (+) HSC/HPC aging during the serial transplantation. Rg1 's anti-aging effect is superior to its effect of treating aging.