Protective effect of Pinus massoniana bark extracts against cisplatin-induced nephrotoxicity in human embryonic kidney cells.
- Author:
Dong-Ru FENG
1
;
Qing-Chen DAI
2
;
Heng XIE
3
;
Ruo-Da LI
3
;
Guang-Yao ZHENG
3
;
Jin-Fa WANG
2
;
Hong-Bin WANG
2
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antioxidants; metabolism; Cisplatin; toxicity; Epithelial Cells; drug effects; enzymology; metabolism; Glutathione; metabolism; Glutathione Peroxidase; metabolism; HEK293 Cells; Humans; Kidney; drug effects; enzymology; metabolism; Malondialdehyde; metabolism; Mice; Pinus; chemistry; Plant Bark; chemistry; Plant Extracts; pharmacology; Protective Agents; pharmacology; Reactive Oxygen Species; metabolism; Superoxide Dismutase; metabolism; Thioredoxin-Disulfide Reductase; metabolism
- From: China Journal of Chinese Materia Medica 2013;38(17):2868-2873
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the in vitro protective effect of Pinus massoniana bark extracts (PMBE) against cisplatin-induced nephrotoxicity in human embryonic kidney cells (HEK293), and preliminarily study its mechanism.
METHODHuman embryonic kidney cells (HEK293) were cultured in vitro. The MTT assay was adopted to test the effect of PMBE and cisplatin on growth of HEK293 cells, and the protective effect of PMBE on cisplatin-induced nephrotoxicity of HEK293, and then detect the intracellular reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH) content, catalase (CAT), superoxide dismutase (SOD) and activity of thioredoxin reductase (TrxR).
RESULTPMBE could promote growth of HEK293 cells at low concentrations, but generate slight nephrotoxicity at high concentration. Cisplatin could inhibit growth of HEK293 cells, increase ROS and MDA content, while reducing SOD, CAT and TrxR. The pre-protective PMBE was added to reduce cisplatin's injury to HEK293 cells, ROS, MDA and GSH content, SOD, CAT and TrxR within certain range.
CONCLUSIONPMBE at specific concentration has the protective effect in cisplatin-induced nephrotoxicity in HEK293 cells. Its mechanism may be related to PMBE's antioxidant activity.
