Effect of shuangshen ningxin formula on energy metabolism of myocardial ischemia/reperfusion rats.
- Author:
Xue-Li LI
1
;
Jian-Xun LIU
2
;
Yu-Jie GUO
2
Author Information
- Publication Type:Journal Article
- MeSH: Adenosine Diphosphate; metabolism; Adenosine Monophosphate; metabolism; Animals; Creatine Kinase, MB Form; blood; Drugs, Chinese Herbal; administration & dosage; Energy Metabolism; drug effects; Humans; Male; Myocardial Ischemia; drug therapy; metabolism; surgery; Myocardial Reperfusion; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2013;38(17):2874-2877
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Shuangshen Ningxin (SSNX) formula on energy metabolism of myocardial ischemia/reperfusion rats.
METHODThe myocardial ischemia/reperfusion model of Wistar rats was established through the ligation of left anterior descending branch of coronary artery of for 40 min and the reperfusion for 2 h. The Wistar rats were randomly divided into six groups: the sham operation group, the model group, the Trimetazidine group (10 mg x kg(-1)) and SSNX groups (22.5, 45, 90 mg x kg(-1)). Preventive administration was conducted for 5 d. The operation was performed at 1 h on the day of the last administration. CK-MB assay kit was adopted to detect the activity of serum CK-MB. HPLC was used to determine ATP, ADP and AMP contents in myocardial tissues and calculate TAN and EC.
RESULTThe preventive administration with SSNX could reduce the activity of serum CK-MB and increase ATP content and EC level in myocardial tissues (P < 0.01 or P < 0.05 vs. the model group).
CONCLUSIONSSNX formula can maintain energy charge in cardiomyocytes and relieve ischemia/reperfusion injury by preserving ischemic myocardium ATP.
