Simultaneous determination of five organic acids in Kudiezi injection by HPLC.
- Author:
Rui LIU
1
;
Lu SUN
2
;
Wen ZHAO
2
;
Yu-Bo LI
2
;
Yan-Jun ZHANG
2
Author Information
1. College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China. lr_8000@163.com
2. College of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
- Publication Type:Journal Article
- MeSH:
Caffeic Acids;
analysis;
Chlorogenic Acid;
analysis;
Chromatography, High Pressure Liquid;
methods;
Coumaric Acids;
analysis;
Drugs, Chinese Herbal;
chemistry;
Quinic Acid;
analogs & derivatives;
analysis;
Succinates;
analysis
- From:
China Journal of Chinese Materia Medica
2013;38(19):3287-3290
- CountryChina
- Language:Chinese
-
Abstract:
The aim was to develop a high performance liquid chromatography method for simultaneous determination of five organic acids in Kudiezi injection. The Diamonsil C18 column (4.6 mm x 200 mm, 5 microm) was adopted with acetonitrile and water as the mobile phase at a gradient mode program. The flow rate was 1.0 mL min-1 , detection wavelength was 325 nm, and column temperature was 35 degree C. The linear range of monocaffeyltartaric acid, chlorogenic acid, caffeic acid, ferulic acid, and chicoric acid were 0. 64-81.60 (r =0. 999 9),0.09-11. 10 (r =0.999 8) ,0.09-11.30 (r =0. 999 8),0.10-12.80 (r =0.999 9),0.43-55. 50 mg L-1 (r = 0.999 8) , respectively. The average recoveries were 101.8% ,100. 9% ,99. 24% ,99. 83% ,101.9%, respectively, with RSD of less than 2.0%. The developed HPLC method was simple, sensitive and accurate with good repeatability. This work provided helpful information for comprehensive quality control of Kudiezi injection. [Key words] Kudiezi injection; organic acids; content determination; HPLC
