Determination of chrysosplenetin, metabolic inhibitor of artemisinin, in rat plasma by UPLC-ms/MS and study on its pharmacokinetics.
- Author:
Jing CHEN
1
;
Hong-Yan JI
1
;
Shi-Jie WEI
2
;
Xue-Yan FU
1
;
Meng-Yingi LIU
1
;
Xiu-Li WU
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Artemisinins; antagonists & inhibitors; Chromatography, High Pressure Liquid; methods; Female; Flavonoids; blood; pharmacology; Male; Rats; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; methods; Tandem Mass Spectrometry
- From: China Journal of Chinese Materia Medica 2013;38(19):3363-3367
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe study aimed to develop the assay of chrysosplenetin (CHR), a metabolic inhibitor of artemisinin by UPLC-MS/MS in rat plasma and investigate the pharmacokinetics parameters of CHR.
METHODThe plasma samples were precipitated by acetonitrile to remove the proteins. Separation was carried out on a Shim-pack XR-ODS C,18(2. 0 mm x 100 mm, 2. 2 micromp.m) column using a mobile phase containing methanol-0. 1% formic acid (87:13) using by diazepam as internal standard. Mass spectrometer with electrospray ionization (ESI) operated in the positive ion mode was used for analysis. Total analysis time was 2 min.
RESULTThe assay was linear in the range 5-5 000 microg L-1 (r =0. 999 3) with recoveries in the range from 69. 0% to 81.2% and satisfied inter-, intra- precision and accuracy. CHR after oral administration is not easy to absorb with double or multimodal peak phenomenon. The t1/2 of CHR after intravenous injection was very short and that of low, medium, and high dosage was (17. 01 +/- 8. 06) , (24. 62 +/- 4. 59), (28. 46+/- 4. 63) min, respectively.
CONCLUSIONThe developed method was special, rapid, and sensitive for determination of CHR pharmacokinetics. [Key words] UPLC-MS/MS; chrysosplenetin; pharmacokinetics; plasma; rat
