C-reactive protein induced human endothelium cells apoptosis is associated with Bcl-2/Bax gene expression changes.
- Author:
Xing-wei ZHANG
1
;
Jun-bo GE
;
Hao PAN
;
Yan GAO
;
Pei-zhang LI
;
Ning-fu WANG
;
Liang ZHOU
;
Jian-min YANG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; C-Reactive Protein; pharmacology; Cell Line; Endothelial Cells; cytology; metabolism; Humans; Proto-Oncogene Proteins c-bcl-2; biosynthesis; Reverse Transcriptase Polymerase Chain Reaction; Umbilical Veins; cytology; bcl-2-Associated X Protein; biosynthesis
- From: Chinese Journal of Cardiology 2006;34(7):639-642
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEIn the present study, we examined the expression changes of Bcl-2/Bax in C-reactive protein (CRP) treated human endothelium cells in vitro.
METHODSThe human umbilical vein endothelial cells (HUVEC) were cultured by digest method for 2 - 3 posterities and incubated with human CRP (0, 1, 5, 25 mg/L for 24 hours) and analyzed by flow cytometer for apoptosis ratio. The effects of CRP in various concentrations on Bcl-2/Bax mRNA and protein expression were examined by RT-PCR and Western Blotting.
RESULTSApoptosis ratio increased, downregulated Bcl-2 (gene promoting cell survival) and upregulated Bax (gene promoting apoptosis) at mRNA and protein levels in proportion to increased CRP concentrations.
CONCLUSIONThese results demonstrate that Bcl-2/Bax could be regulated by CRP in human HUVECs and might play a causal role in CRP-induced apoptosis.
