siRNA inhibits efgl7 expression in human endothelial cell line HUVEC.
- Author:
Wei-dong JIANG
1
;
Ji-ping ZENG
;
Ai-qiong QIN
;
Qing-hua LU
;
Dong-ling XU
;
Zhi-ming GE
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Endothelial Cells; metabolism; Epidermal Growth Factor; biosynthesis; genetics; Gene Expression; Humans; RNA, Small Interfering; genetics; Transcription, Genetic; Umbilical Veins; cytology
- From: Chinese Journal of Cardiology 2006;34(7):643-646
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effects of small interference RNA (siRNA) on epidermal growth factor-like domain 7 (egfl7) gene expression in human endothelial cell line HUVEC.
METHODSsiRNA targeting egfl7 (siRNA1, siRNA2, siRNA3 and siRNA4) was constructed through online design of Amnion company and transfected into human endothelial cell line HUVEC with lipofectamine. The nontransfected cells and cells treated with control siRNA were taken as controls. At 24, 48 and 72 hours post various interventions, cell viability was determined by MTS method as well as LDH and ATP releasing tests. egfl7 expressions at protein and mRNA levels were detected by Western blot and RT-PCR respectively.
RESULTSCell survival rate, LDH and ATP release were significantly reduced in siRNA treated cells compared to control cells (P < 0.05). Similarly, egfl7 expression at protein and mRNA levels was also significantly reduced in siRNA treated cells (P < 0.01), especially in siRNA1 treated cells.
CONCLUSIONsiRNA inhibited egfl7 gene expression and cell survival in HUVEC.
