Nitroglycerin tolerance aggravates arterial ischemia/reperfusion injury by increasing nitrotyrosine and ONOO- production.
- Author:
Qian FAN
1
;
Lin ZHANG
;
Feng GAO
;
Xin-liang MA
;
Xue-bin CAO
;
Xin-chun YANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aorta; drug effects; Drug Tolerance; Glutathione; metabolism; In Vitro Techniques; Male; Nitroglycerin; pharmacology; Rats; Rats, Sprague-Dawley; Reperfusion Injury; pathology; physiopathology; Vasoconstriction
- From: Chinese Journal of Cardiology 2006;34(8):680-684
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of nitroglycerin tolerance (NT) on arterial ischemia (90 min) and reperfusion (120 min).
METHODSMale Sprague-Dawley rats were infused with nitroglycerin (GTN) or saline for 12 h and ascending aorta was rapidly isolated. The isolated aorta was subjected to one of the following treatments: stimulative ischemia/reperfusion, stimulative ischemia/reperfusion (I/R) plus glutathione (GSH, 0.1 mmol/L) during reperfusion, or control solution (Kreb's solution for 3.5 h).
RESULTSCompared with I/R group, contractile function, vasorelaxation responses to Ach, NO production were significantly decreased and CK, LDH activity as well as nitrotyrosine formation in reperfusion solution were significantly increased in I/R + NT group and these effects could be prevented with addition of GSH in I/R + NT aortas.
CONCLUSIONSOur results demonstrated that NT could aggravate arterial I/R injury by increasing the production of ONOO- and GSH may play a cardioprotective role against NT-induced myocardial injury by attenuating the formation of ONOO-.
