Association between calcium-sensing receptor protein expression and rat cardiomyocyte apoptosis.
- Author:
Yi-Hua SUN
1
;
Chang-qing XU
;
Hong LI
;
Sa SHI
;
Wei-hua ZHANG
;
Ya-jun ZHAO
;
Yan-qiao ZHANG
;
Wei-min HAN
;
Li-ping HAN
;
Chun-ming JIANG
;
Quan-feng LI
;
Rui WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Caspase 3; metabolism; Female; Male; Myocardium; metabolism; Myocytes, Cardiac; metabolism; RNA, Messenger; biosynthesis; Rats; Rats, Wistar; Receptors, Calcium-Sensing; biosynthesis; genetics; Signal Transduction
- From: Chinese Journal of Cardiology 2006;34(8):739-743
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between calcium-sensing receptor protein (CaSR) expression and rat cardiomyocyte apoptosis and related signal transduction pathways.
METHODSThe CaSR, BCl2, Caspase3 protein and ERK1/2 phosphorylation or non-phosphorylation were detected by Western blot. Cardiomyocyte apoptosis was detected by flow cytometry and immunofluorescence.
RESULTSCaSR protein was detected in rat cardiac tissue and CaSR activator gadolinium (GdCl3) induced cardiomyocyte apoptosis and increased ERK1/2 phosphorylation and expression of BCl2 and activated Caspase3. The selective mitogen-activated protein kinase (MAPK) inhibitor PD98059 abolished gadolinium -induced ERK1/2 activation and BCl2 expression, further increased the activation of Caspase3 and cardiomyocyte apoptosis.
CONCLUSIONOur results demonstrate the CaSR existence in cardiomyocytes and CaSR activation by gadolinium can induce myocyte apoptosis by activating Caspase3 and tyrosine protein kinase pathway.
