Role of AcSDKP on collagen synthesis and degradation in cultured rat cardiac fibroblast.
- Author:
Fang YANG
1
;
Xi-ling ZHU
;
Li-ping WANG
;
Xu-dong SONG
;
Rui-min WANG
;
Zhi-guo LI
;
Ling LUO
;
Wan-mi HU
;
Wen-dong MA
;
Xin PEI
;
Li-juan ZHANG
;
Qi-jia LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Cells, Cultured; Collagen; biosynthesis; Fibroblasts; drug effects; metabolism; Matrix Metalloproteinase 2; biosynthesis; Matrix Metalloproteinase 9; biosynthesis; Myocytes, Cardiac; drug effects; metabolism; Oligopeptides; physiology; Platelet-Derived Growth Factor; drug effects; Rats; Rats, Wistar
- From: Chinese Journal of Cardiology 2006;34(9):843-846
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of AcSDKP on collagen synthesis and degradation in cultured rat cardiac fibroblasts.
METHODSNeonatal rat cardiac fibroblasts were isolated and stimulated by PDGF. The cell proliferation was observed by (3)H-TdR incorporation assay. The synthesis of collagen was measured by (3)H-proline incorporation assay. The expression of type I and type III collagen and MMP-1 protein were measured by Western blot. The MMP-2 and MMP-9 activity was evaluated with zymography assay.
RESULTSPDGF stimulated cardiac fibroblasts proliferation with increased collagen synthesis and type I and type III collagen protein expressions as well as MMP-2 and MMP-9 activities and MMP-1 expression. AcSDKP inhibited cardiac fibroblasts proliferation induced by PDGF and reduced collagen synthesis and type I and type III collagen protein expression. AcSDKP also further up-regulated MMP-2 and MMP-9 activities and MMP-1 expression in cardiac fibroblasts induced by PDGF.
CONCLUSIONAcSDKP inhibited proliferation and collagen synthesis and up-regulated matrix metalloproteinases activity or expression induced by PDGF, which was possibly related with the effect of AcSDKP anti-fibrosis.
