Porcine MSC-derived CLC induced by rabbit's cardiomyocytes extract did not induce immune reaction in vivo.
- Author:
Xin-Xin CAO
1
;
Yu-Hua DAI
;
Shu-Yang ZHANG
;
Lian-Feng CHEN
;
Jin-Zhi LAI
;
Xiao-Wei YAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; Bone Marrow Cells; immunology; Bone Marrow Transplantation; immunology; CD4 Antigens; immunology; CD4-CD8 Ratio; CD8 Antigens; immunology; Female; Male; Myocytes, Cardiac; cytology; Rabbits; Swine; Swine, Miniature
- From: Chinese Journal of Cardiology 2006;34(10):929-931
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the in vivo immune reaction of transplanting porcine MSC-derived CLC with rabbit cardiomyocytes extracts induced differentiation or in vitro cultured porcine MSC.
METHODSAfter injecting the MSC-derived CLC or MSC to the original porcines, the number of CD4+, CD8+ T cells were determined by flow cytometry. The serum concentrations of IL-2, IL-4 were measured by ELISA, and the porcine spleen lymphocyte CTL cytotoxicity was determined by Cell Counting Kit-8 Assay.
RESULTSThe numbers of CD4+ and CD8+ T cells, the serum concentrations of IL-2, IL-4 and spleen lymphocyte CTL cytotoxicity were all similar in porcines received MSC-derived CLC induced by rabbit's CMs extract or MSC transplantation.
CONCLUSIONThe porcine MSC-derived CLC induced by rabbit's CMs extract did not induce extra immune reaction when injected back to the original porcine.
