Fosinopril but not metoprolol attenuated the increased matrix metallo-proteinases 9 expression at mRNA and protein levels of human umbilical vein endothelial cells exposed to oscillatory flow.
- Author:
Wen-Li CHENG
1
;
Yuan-Nan KE
;
Yong WANG
;
Li CHEN
;
Shu-Ying FAN
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Endothelial Cells; drug effects; metabolism; Endothelium, Vascular; Fosinopril; pharmacology; Humans; Matrix Metalloproteinase 9; metabolism; Metoprolol; pharmacology; RNA, Messenger; metabolism; Stress, Mechanical; Umbilical Veins; cytology
- From: Chinese Journal of Cardiology 2006;34(12):1113-1116
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of fosinopril and metoprolol on metalloproteinases 9 (MMP9) expression of human umbilical vein endothelial cells (HUVECs) stimulated by oscillatory flow.
METHODSHUVECs were exposed to steady laminar flow or oscillatory flow, laminar flow or oscillatory flow plus various concentrations (1 x 10(-7) mol/L, 1 x 10(-5) mol/L) of fosinopril and metoprolol for 4 and 24 hours. MMP9 mRNA and protein expressions of HUVECs were determined by RT-PCR and Western blot, respectively.
RESULTSMMP9 expression at mRNA and protein levels were significantly increased in HUVECs exposed to oscillatory flow than that to laminar flow and these could be down-regulated by coincubation with fosinopril (1 x 10(-7) mol/L, 1 x 10(-5) mol/L, P < 0.01, P < 0.05, respectively) but not by co-incubation with metoprolol.
CONCLUSIONFosinopril can attenuate the increased MMP9 expression at mRNA and protein levels of HUVECs exposed to oscillatory flow.
