Effects of recombinant adenovirus encoding human apM1 gene on proliferation and nitric oxide synthase activity in human umbilical vein endothelial cells.
- Author:
Bing-Rong LI
1
;
Dan ZHENG
;
Hua-Cong DENG
;
Jin-Bo LIU
;
Li-Zhen LAN
;
Hong-Ting ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Adiponectin; genetics; Cell Proliferation; Cells, Cultured; Endothelial Cells; cytology; metabolism; Endothelium, Vascular; cytology; Gene Expression; Gene Transfer Techniques; Humans; Nitric Oxide Synthase; metabolism; Umbilical Veins; cytology
- From: Chinese Journal of Cardiology 2006;34(12):1122-1125
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effects of recombinant adenovirus encoding human apM1 gene on proliferation and nitric oxide synthase (NOS) activity in human umbilical vein endothelial cells (HUVECs).
METHODSProtein expression of apM1 in cell culture supernatant of HUVECs transfected with human Ad-apM1 was detected by double antibody sandwich ELISA. The effect of human adiponectin on cell proliferation was assessed by MTT assay. The total NOS and iNOS expressions were measured by chromatometre.
RESULTSHuman adiponectin protein level and total NOS and eNOS expressions were significant increased and iNOS expression significantly reduced in culture supernatant of HUVECs infected with Ad-apM1 compared to that in control HUVECs. The recombinant adenovirus had no influence on HUVECs growth as determined by MTT assay.
CONCLUSIONSHuman Ad-apM1 can be effectively expressed in HUVECs and do not influence HUVECs growth. Increased total NOS and eNOS expressions and decreased iNOS expression in HUVECs transfected with Ad-apM1 gene suggest a potential role of Ad-apM1 gene transfer for the prevention and treatment of arteriosclerosis.
