Cannabinoids increase mechanosensitivity of trigeminal ganglion neurons innervating the inner walls of rat anterior chambers via activation of TRPA1.
- Author:
Yun LING
1
;
Zhuang-Li HU
2
;
Qing-Li MENG
1
;
Peng FANG
2
;
Hai-Xia LIU
3
Author Information
1. Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
2. Department of Pharmacology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
3. Department of Ophthalmology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China. haixia72@aliyun.com.
- Publication Type:Journal Article
- Keywords:
cannabinoids;
intraocular pressure;
mechanosensitive channel;
transient receptor potential ankyrin 1;
trigeminal ganglion
- MeSH:
Action Potentials;
drug effects;
Animals;
Anterior Chamber;
drug effects;
innervation;
Cannabinoids;
administration & dosage;
Eye;
drug effects;
innervation;
Neurons;
drug effects;
Patch-Clamp Techniques;
Rats;
Rats, Sprague-Dawley;
TRPA1 Cation Channel;
TRPC Cation Channels;
biosynthesis;
genetics;
Trigeminal Ganglion;
drug effects;
physiology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2016;36(5):727-731
- CountryChina
- Language:English
-
Abstract:
Our previous study found that some trigeminal ganglion (TG) nerve endings in the inner walls of rat anterior chambers were mechanosensitive, and transient receptor potential ankyrin 1 (TRPA1) was an essential mechanosensitive channel in the membrane. To address the effect of cannabinoids on the mechanosensitive TG nerve endings in the inner walls of anterior chambers of rat eye, we investigated the effect of the (R)-(+)-WIN55, 212-2 mesylate salt (WIN), a synthetic cannabinoid on their cell bodies in vitro. Rat TG neurons innervating the inner walls of the anterior chambers were labeled by 1,1'-dilinoleyl-3,3,3',3'-tetramethylindocarbocyanine, 4-chlorobenzenesulfona (FAST DiI). Whole cell patch clamp was performed to record the currents induced by drugs and mechanical stimulation. Mechanical stimulation was applied to the neurons by buffer ejection. WIN evoked inward currents via TRPA1 activation in FAST DiI-labeled TG neurons. WIN enhanced mechanosensitive currents via TRPA1 activation in FAST DiI-labeled TG neurons. Our results indicate that cannabinoids can enhance the mechanosensitivity of TG endings in the inner walls of anterior chambers of rat eye via TRPA1 activation.
