Global protein expression analysis of molecular markers of DS-1-47, a component of implantation-promoting traditional chinese medicine.
- Author:
Yan-Ling LI
1
;
Xiao-Yan ZHANG
1
;
Yu LENG
1
;
Yan-Li WU
1
;
Jing LI
2
;
Yun-Xia WU
3
Author Information
1. Department of Traditional Chinese Medicine, School of Pharmacy, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, 430030, China.
2. Department of Emergency, Hubei General Hospital of Chinese People's Armed Police Forces, Wuhan, 430061, China. 1150790777@qq.com.
3. Department of Traditional Chinese Medicine, School of Pharmacy, Tongji Medical School, Huazhong University of Science and Technology, Wuhan, 430030, China. wuyunxia@hust.edu.cn.
- Publication Type:Journal Article
- Keywords:
DS-1-47;
LCM-DE-MS;
promoting implantation;
proteomics
- MeSH:
Animals;
Drugs, Chinese Herbal;
pharmacology;
Embryo Implantation;
drug effects;
Female;
Mice;
Ovulation Induction;
Pregnancy;
Proteome;
genetics;
metabolism;
Uterus;
drug effects;
metabolism;
physiology
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2016;36(6):910-915
- CountryChina
- Language:English
-
Abstract:
This study investigated the molecular markers of DS-1-47, a component of an implantation- promoting traditional Chinese medicine consisting of Astragalus mongholicus, Atractylodes macrocephala, Scutellaria baicalensis and Dipsacales, in an attempt to clarify the molecular mechanism and action targets of DS-1-47. Controlled ovarian stimulation (COS) method was used to establish the implantation dysfunction models of mice. Animals were divided into normal pregnant group, COS model group and DS-1-47 group. Laser capture microdissection-double dimensional electrophoresis-mass spectrum (LCM-DE-MS) was used to analyze the uterine protein molecules that were possibly involved in the promotion of implantation. Twenty-three proteins in DS-1-47 group were significantly changed as compared to those in COS model group, with 7 proteins down-regulated and 16 proteins up-regulated. Except for some constituent proteins, the down-regulated proteins included collagen α-1 (VI) chain, keratin 7, keratin 14, myosin regulatory light chain 12B, myosin light polypeptide 9, heat shock protein β-7, and C-U-editing enzyme APOBEC-2; the up-regulated proteins included apolipoprotein A-I, calcium regulated protein-3, proliferating cell nuclear antigen, L-xylulose reductase, and calcium binding protein. These 23 proteins that were regulated by DS-1-47 represented a broad diversity of molecule functions. The down-regulated proteins were associated with stress and immune response, and those up-regulated proteins were related to proliferation. It was suggested that these proteins were important in regulating the uterine environment for the blastocyst implantation. By identification of DS-1-47 markers, proteomic analysis coupled with functional assays is demonstrated to be a promising approach to better understand the molecular mechanism of traditional Chinese medicine.
