Targeting BCRP/ABCG2 by RNA Interference Enhances the Chemotherapy Sensitivity of Human Colon Cancer Side Population Cells
10.1007/s11596-017-1720-1
- Author:
LI JIAN
1
Author Information
1. Department of Lymphoma
- Keywords:
side population cells;
colon cancer;
chemotherapy sensitivity;
RNAi,bcrp/abcg2
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2017;37(2):231-236
- CountryChina
- Language:Chinese
-
Abstract:
Relapse and metastasis are frequent in colon cancer and may be linked to stem cell characteristics.This study isolated side population (SP) cells from a colon cancer cell line (Colo-320) and examined their self-renewal and differentiation abilities.Compared to non-SP (NSP) cells,SP colon cancer cells were more tumorigenic in vivo and exhibited more invasive characteristics and a greater ability to form colonies.Additionally,more cells were in G0/G1 phase and more highly expressed the multidrug resistance protein BCRP/ABCG2.We achieved enhanced chemotherapy sensitivity by transfecting SP cells with a hairpin-like,small interfering RNA (siRNA) eukaryotic expression plasmid targeting BCRP/ABCG2.
