Cytotoxicity study of heparin coated polyvinyl chloride material.
- Author:
Jian YANG
1
;
Dinghua YI
;
Jincheng LIU
;
Jian LIU
;
Ruijun CAO
;
Feng QIN
Author Information
1. Center of Cardiovascular Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an 710033, China. yangjian1212@hotmail.com
- Publication Type:Journal Article
- MeSH:
Animals;
Cells, Cultured;
Coated Materials, Biocompatible;
toxicity;
Fibroblasts;
cytology;
Heparin;
toxicity;
Materials Testing;
Mice;
Polyvinyl Chloride;
toxicity
- From:
Journal of Biomedical Engineering
2005;22(4):739-741
- CountryChina
- Language:Chinese
-
Abstract:
In this study, heparin ionically coated polyvinyl chloride (PVC) material was prepared by heparin-benzalkonium chloride complex (Group A), heparin-benzalkonium bromide complex (Group B) and heparin-polyethyleneimine compound (Group C). Cytotoxicity evaluation was conducted by direct cell contact evaluation and MTT colorimetry method. The results showed Group A and Group B caused L-929 cells to die out while Group C showed good compatibility with cells. The OD levels of Group A and B were lower than that of the Group C in MTT test. Method A and method B of heparin coating had remarkable cytotoxicity, while method C had little cytotoxicity and could be further studied for clinical use.
