Construction of the recombinant adenovirus carrying sodium/iodide symporter gene.
- Author:
Rui HUANG
1
;
An'ren KUANG
;
Haifang YU
;
Chao MA
;
Gongshun TANG
Author Information
1. Department of Nuclear Medicine, West China Hospital, Sichuan University, Chengdu, China.
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
metabolism;
DNA, Complementary;
genetics;
Genetic Vectors;
genetics;
metabolism;
Graves Disease;
genetics;
Green Fluorescent Proteins;
genetics;
metabolism;
Humans;
Recombinant Fusion Proteins;
biosynthesis;
genetics;
Symporters;
biosynthesis;
genetics
- From:
Journal of Biomedical Engineering
2005;22(4):765-768
- CountryChina
- Language:Chinese
-
Abstract:
Human Sodium/Iodide symporter gene cDNA was amplified from thyroid tissue of the patient suffering from Graves disease by RT-PCR, and T/A cloned into pGEM-TEasy-NIS for sequencing, subcloned into shuttle plasmid pAdTrack-CMV which contained a green fluorescent protein (GFP) gene, and then forwarded to homologous recombinant in the bacteria BJ5183 that already contained AdEasy-1 plasmid. Positive recombinant adenovirus vector was selected, packaged and amplified in the 293 cells to obtain recombinant adenovirus. The results showed that the recombinant AdNIS was correctly constructed and confirmed by restriction enzyme analysis and PCR. The viral titer was 2. 5 - 3 x 10(9) efu/ml. So, the recombinant adenovirus vector carrying hNIS was successfully constructed, thus providing a basis for researches on 131I therapy in nonthyroid carcinoma.
