Construction of recombinant human smad 7 adenoviral vector and its expression in vitro.
- Author:
Danru WANG
1
;
Wei LIU
;
Xiaoli WU
;
Juanjuan WU
;
Junhong LU
;
Deli LIU
;
Yilin CAO
;
Disheng ZHANG
Author Information
1. Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Second Medical University, Shanghai, China. wangdanru@hotmail.com
- Publication Type:Journal Article
- MeSH:
Adenoviridae;
genetics;
metabolism;
Cells, Cultured;
Fibroblasts;
metabolism;
pathology;
Genetic Vectors;
genetics;
metabolism;
Humans;
Keloid;
metabolism;
pathology;
RNA, Messenger;
biosynthesis;
genetics;
Recombinant Fusion Proteins;
biosynthesis;
genetics;
Smad7 Protein;
biosynthesis;
genetics;
Transfection
- From:
Journal of Biomedical Engineering
2005;22(4):769-772
- CountryChina
- Language:Chinese
-
Abstract:
The recombinant human Smad7 adenoviral vector was constructed by direct DNA cloning protocol and then transfected into 293 cells for virus packaging. After amplification and purification, the recombinant adenovirus was used to infect the keloid fibroblasts. The Smad7 mRNA transcription of the infected cells was detected by RT-PCR. The recombinant Adeno-Smad7 was correctly constructed and confirmed by both restriction analysis and PCR analysis. RT-PCR showed the over expression of adenovirus mediated Smad7 mRNA in keloid cells. These results demonstrated that the recombinant Smad7 adenoviral vector can be expressed in cultured cells in vitro, and it may provide a new therapeutic strategy for keloid gene therapy.
