Effects of triptolide on anti-angiogenesis by reducing expression of urokinase plasminogen activator.
- Author:
Yi DING
1
;
Jiancheng ZHANG
;
Lijun HOU
;
Jie ZHANG
;
Qiulin WANG
;
Shuren WANG
Author Information
1. Department of Pathophysiology, West China School of Basic Medical Sciences and Forensic Medicine, Sichuan University, Chengdu, China.
- Publication Type:Journal Article
- MeSH:
Angiogenesis Inhibitors;
pharmacology;
Animals;
Cells, Cultured;
Chick Embryo;
Chorioallantoic Membrane;
blood supply;
Diterpenes;
pharmacology;
Down-Regulation;
drug effects;
Endothelial Cells;
cytology;
metabolism;
Epoxy Compounds;
pharmacology;
Humans;
Phenanthrenes;
pharmacology;
RNA, Messenger;
biosynthesis;
genetics;
Umbilical Veins;
cytology;
metabolism;
Urokinase-Type Plasminogen Activator;
biosynthesis;
genetics
- From:
Journal of Biomedical Engineering
2005;22(4):778-781
- CountryChina
- Language:Chinese
-
Abstract:
In order to unveil the anti-angiogenic mechanism of triptolide, we investigated the effects of triptolide on the proliferation, migration, angiogenesis and u-PA expression of cultured HUVECs. MTT assay found that triptolide could inhibit the proliferation of HUVECs, and three-dimensional culture system revealed that triptolide could curb the migration of HUVECs. The chick embryo chorioallantoic membrane (CAM) test showed that triptolide could inhibit angiogenesis. Real time quantitive RT-PCR showed the expression of u-PA of HUVECs was down-regulated by triptolide. Therefore, triptolide may inhibit the proliferation and migration of endothelial cell by way of reducing the expression of u-PA. These findings may conduce to the elucidation of the anti-angiogenic mechanism of triptolide.
