The role of caveolae in shear stress-induced endothelial nitric-oxide synthase activation.
- Author:
Yinping LI
1
;
Jingping OUYANG
;
Hanqiao ZHENG
;
Zhui YU
;
Baohua WANG
Author Information
1. Faculty of Pathology and Pathophysiology, Wuhan University, Wuhan 430071, China.
- Publication Type:Journal Article
- MeSH:
Caveolae;
physiology;
Cells, Cultured;
Endothelium, Vascular;
cytology;
Filipin;
pharmacology;
Humans;
Nitric Oxide Synthase Type III;
metabolism;
Shear Strength;
Umbilical Veins;
cytology
- From:
Journal of Biomedical Engineering
2005;22(5):1020-1023
- CountryChina
- Language:Chinese
-
Abstract:
This article deals with the influence of shear stress on endothelial NO synthesis, and the role of caveolae in shear stress-induced eNOS activation. Human umbilical vascular endothelial cells (HUVEC) were cultured and exposed to different levels of laminal shear stress and Filipin, the perfused cultures were collected, and NO(2-)/NO(3-) was detected using nitrate reduction method. The structure of caveolae was observed through transmission electron microscopy (TEM). The level of NO(2-)-/NO(3-) was found to increase with the elevation of shear stress level (P < 0.01). It was the highest at 1.5 N/m2. After treatment with Filipin, the level of NO produced by HUVEC decreased significantly (P < 0.01), but after recovery and shear without Filipin, the level of NO synthesis bounded back (P < 0.01). It was then concluded that shear stress can induce endothelial NO synthesis and caveolae plays a key role in shear stress-induced eNOS activation.
