Synthesis of peptide fragment of melittin and the function of rheumatoid arthritis cure.
- Author:
Meili YU
1
;
Yong WANG
;
Xiaohua LI
;
Shuchang FANG
;
Junchen XUE
;
Jijing SONG
Author Information
1. Hepato-biliary Disease Institute, Tianjin The Third Center Hospital, Tianjin 300170, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Arthritis, Rheumatoid;
therapy;
Melitten;
chemical synthesis;
therapeutic use;
Peptide Fragments;
chemical synthesis;
therapeutic use;
Rabbits
- From:
Journal of Biomedical Engineering
2005;22(5):1031-1035
- CountryChina
- Language:Chinese
-
Abstract:
To retain the anti-rheumatoid arthritis activity of melittin and to reduce the hemolysis and hypersusceptibility caused by melittin, a deletion peptide of melittin was synthesized. Its ant-inflammation effect was observed . A hydrophile peptide fragment of melittin was synthesized by standard solid-phase method. The product was analyzed by HPLC and MS. The relevant hemolysis and hypersusceptibility were tested. The rabbits' model of immune arthritis were established and treated. The results showed that the hemolysis rate for peptide fragment was less than 5%, the hypersusceptibility rate was less than 8%. The hydrophile peptide fragment of melittin may retain anti-rheumatoid arthritis activity and reduce the melittin-induced hemolysis and hypersusceptibility.
