Correlation between mutation of GyrA and ParC genes and quinolone resistance in Neisseria gonorrhoeae.
- Author:
Ping XIE
1
;
Zu-Huang MI
;
Qin LI
;
Ji ZHANG
;
Chen-Yue XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; DNA Gyrase; genetics; DNA Topoisomerase IV; genetics; Drug Resistance, Bacterial; genetics; Humans; Molecular Sequence Data; Mutation; Neisseria gonorrhoeae; drug effects; genetics; isolation & purification; Quinolones; pharmacology
- From: National Journal of Andrology 2003;9(4):275-278
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo investigate the correlation between mutation of GyrA and ParC genes and quinolone resistance in Neisseria Gonorrhoeae.
METHODSThe gene fragments of quinolone resistance-determining region (QRDR) in GyrA and ParC genes in 20 Neisseria gonorrhoeae strains clinically isolated in Wuxi, China, were sequenced, and the susceptibility of the 20 strains to quinolone was examined by agar diffusion method.
RESULTSThe mutations at the Asp95 point in GyrA gene were found in 20 strains. Of the 19 stains examined, 16 had mutations at the 86, 87, 88, 91 points in ParC genes.
CONCLUSIONSThe mutations of Asp95 in GyrA gene and Asp86, Ser87, Ser88, Glu91 in ParC gene contribute to quinolone resistance in Neisseria Gonorrhoeae.
