Effect of immunological orchitis on sperm specific enzyme and fertility.
- Author:
Lei CHEN
1
;
Wei-Ping XIA
;
Zhi-Heng ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Acrosin; metabolism; Animals; Autoimmune Diseases; enzymology; pathology; physiopathology; Disease Models, Animal; Fertility; physiology; Guinea Pigs; L-Lactate Dehydrogenase; metabolism; Male; Orchitis; enzymology; pathology; physiopathology; Spermatozoa; enzymology; pathology; Testis; pathology
- From:National Journal of Andrology 2003;9(5):344-346
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of immunological orchitis on spermatic specific enzyme and fertility.
METHODSExperimental allergic orchitis (EAO) model of guinea pigs was duplicated. The histological and morphological changes of spermatic acrosomal protease and hyaluronidase, lactate dehydrogenase, sperm in epididymis and testes were observed by means of enzyme kinetical spectrophotometry and gelatin fixation of substrate thin membrane.
RESULTSThe activity of acrosomal protease, hyaluronidase and spermatic cytoplasmic lactic dehydrogenase in the epididymis acrosomal enzyme system became low, and so did the quality of sperm in epididymis. Remarkable morphological changes of spermatogenic cells developed in the convoluted seminiferous tubules.
CONCLUSIONSEAO remarkably affects the fertility of male guinea pigs. The orchis and epididymal sperms might be the sites of action.
