Molecular cloning and identification of calmegin gene in embryo and adult testes by cDNA microarray hybridization.
- Author:
Ting CUI
1
;
Hui ZHU
;
Li-Rong WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Base Sequence; Calcium-Binding Proteins; chemistry; genetics; Cloning, Molecular; Gene Expression Regulation, Developmental; Humans; Male; Molecular Chaperones; chemistry; genetics; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; Spermatogenesis; genetics; Testis; embryology; metabolism
- From: National Journal of Andrology 2003;9(5):355-360
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo identify gene differential expression in embryo and adult testes by cDNA microarray techniques.
METHODScDNA probes of embryo and adult testes were used to hybridize the cDNA microarray of human testis, and the clones of differential hybridization were sequenced and analyzed.
RESULTSThe calmegin (CLGN) gene which had been confirmed to be involved in spermatogenesis was found, and it exhibited more than 20-fold difference at expression level between adult and embryo human testes.
CONCLUSIONGenes differential expression in adult and embryo human testes can be identified by cDNA microarray hybridization.
