Effects of mTR antisense oligodeoxynucleotide on telomerase activity and the expression of telomerase mTR in type A spermatogonia.
- Author:
Zhe-Wei YE
1
;
Xiao-Chun CHEN
;
Shu-Hua YANG
;
Long-Jie GU
;
Jiang CHEN
;
Gong-Cheng LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Male; Oligodeoxyribonucleotides, Antisense; pharmacology; RNA; antagonists & inhibitors; genetics; metabolism; RNA, Messenger; analysis; Rats; Rats, Sprague-Dawley; Spermatogonia; cytology; enzymology; ultrastructure; Telomerase; antagonists & inhibitors; genetics; metabolism
- From: National Journal of Andrology 2003;9(6):421-428
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVESTo study the inhibitory effects of mouse telomerase RNA (mTR) antisense oligodeoxynucleotide(ASODN) on telomerase activity in rat spermatogonia.
METHODS9-mer phosphorothioate mTR-ASODN was encapsulated by Lipofect AMINE 2000 (LF 2000) and transfected to type A spermatogonia in Snrague Dawley (SD) rat. Telomerase activity was detected by aid of TRAP-SYBR-Green staining and Bioluminescence technique in type A spermatogonia treated or untreated with ASODN.
RESULTSmTR-ASODN conjugated with LF 2000 could significantly inhibit telomerase activity of spermatogonia(P < 0.01). mTR mRNA level also decreased while the spermatogonia were treated with ASODN for 24 h. No change of telomerase activity and apoptosis were observed when SODN, RODN or single LF 2000 was used.
CONCLUSIONSAntisense oligodeoxynucleotide of mTR conjugated with LF 2000 could significantly inhibit telomerase activity of spermatogonia. mTR-ASODN might inhibit telomerase activity of spermatogonia at transcription level.
