Real-time fluorescent PCR for screening AZFc/DAZ microdeletions on the Y chromosome in male infertility patients.
- Author:
Cong-Yi YU
1
;
Guang-Lun ZHUANG
;
Can-Quan ZHOU
;
Zong-He YAN
;
Wei LI
;
Hua GAO
;
De-Rong RUI
Author Information
- Publication Type:Journal Article
- MeSH: Chromosome Deletion; Chromosomes, Human, Y; Deleted in Azoospermia 1 Protein; Fluorescence; Humans; Infertility, Male; genetics; Male; Polymerase Chain Reaction; methods; RNA-Binding Proteins; genetics
- From:National Journal of Andrology 2003;9(6):436-442
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a real-time fluorescent PCR protocol suitable for the routine screening of AZFc/DAZ microdeletions on the Y chromosome in azoospermic and oligozoospermic male infertility patients.
METHODSA set of real-time fluorescent PCR was established. Eighty-seven azoospermic and ligozoospermic patients undergoing ICSI in the IVF center and 30 azoospermic men undergoing testicular biopsy in the clinic of urology surgery were screened for AZFc/DAZ microdeletions of Y chromosome.
RESULTSEleven cases (9.4%) of AZFc/DAZ microdeletions were found in 117 cases of azoospermic and oligozoospermic patients by screening of realtime fluorescent PCR. Four cases (6.6%) were found in 61 oligozoospermic patients, and 7 cases (12.5%) were found in 56 azoospermic patients.
CONCLUSIONThe real-time fluorescent PCR protocol presented in this study is an easy and reliable method for detection of AZFc/DAZ microdeletions on the Y chromosome, which yields identical results to those of the multiplex PCR.
