Effects of shouwu-huanjing recipe on human sperm motility and fertility in vitro.
- Author:
Jin-Xiong ZENG
1
;
Xi-Hu DAI
;
Jian-Hua LIU
;
Jia-Hui YANG
;
Jin-Feng CHEN
;
Mu-Nan LIN
Author Information
- Publication Type:Journal Article
- MeSH: Acrosome Reaction; Animals; Cricetinae; Fertility; drug effects; Humans; Male; Medicine, Chinese Traditional; Mesocricetus; Rats; Rats, Sprague-Dawley; Sperm Motility; drug effects
- From: National Journal of Andrology 2003;9(6):476-479
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of Shouwu-Huanjing Recipe(SWHJR) medicated serum on human sperm motility and fertility in vitro.
METHODSHuman sperm was co-cultured with SWHJR medicated serum in vitro. Human sperm motility was evaluated by computer-assisted semen analysis(CASA). The acrosome reaction and the capability of penetrating zona-free hamster eggs were also observed.
RESULTSThe co-cultured SWHJR medicated serum significantly increased the sperm motion velocity(VAP, VCL, VSL) (P < 0.01), the amplitude of lateral head movement (ALH) and the beat frequency of flagellum(BCF), the density of progressive motility sperms (P < 0.05), the acrosome reaction rate(P < 0.001), the fertilization rate(FR) and the fertilization index (FI) in sperm penetration assay(SPA) test (P < 0.01). The stimulation of SWHJR medicated serum occurred in dose-dependent manner.
CONCLUSIONSWHJR can improve human sperm motility and fertility.
