Expression of androgen receptor isoforms in normal human prostate.
- Author:
Shu-Jie XIA
1
;
Gang-Yue HAO
;
Wei WEN
;
Jie ZHANG
;
Wei-Guo LI
;
Yi-Yong ZHU
;
Xiao-Da TANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Humans; Isoelectric Focusing; Isoelectric Point; Male; Prostate; metabolism; Protein Isoforms; biosynthesis; Receptors, Androgen; biosynthesis
- From: National Journal of Andrology 2003;9(7):501-506
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of androgen receptor (AR) isoforms in normal human prostate.
METHODSFourteen normal prostatic specimens from donors, aged 25 on average (21-28 yr), were analyzed by high resolution isoelectric focusing (IEF). The expression of AR isoforms was demonstrated in all 14 normal human prostatic tissues.
RESULTSFour types of AR isoforms were detected with isoelectric point value at 6.5, 6.0, 5.8 and 5.3 in 14 prostatic specimens. Binding of 3H-dihydrotestosterone (DHT) to these four AR isoforms was inhibited by the addition of 100-fold excess of DHT and testosterone. No effect of progesterone, estradiol and diethylstilbestrol on tritiated hormone binding was observed.
CONCLUSIONSThere are four AR isoforms in normal human prostate. The expression of AR isoforms is different from one another.
