Studies on dihydrotestosterone-binding capacity in prostate tissue.
- Author:
Hong-Tao JIANG
1
;
Zhao-Dian CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Cell Nucleus; metabolism; Cytoplasm; metabolism; Dihydrotestosterone; metabolism; Humans; Male; Prostate; metabolism; Protein Binding
- From: National Journal of Andrology 2003;9(8):589-593
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of the binding ability of the dihydrotestosterone(DHT) in prostate.
METHODSTwenty-two normal prostate tissues taken from accident-death corpses without serious diseases, and cytosolic and nuclear fractions were prepared with all the endogenous hormone removed from the cytosolic and nuclear fractions by ether stripping. The content of the bound 3H-DHT was assayed by adding 3H-DHT.
RESULTSThe average DHT-binding capacity of the DHT-binding protein in prostate was (0.0263 +/- 0.0047) nmol/g wet tissue. The DHT-binding capacities of cytosolic and nuclear fractions were (0.0103 +/- 0.0015) nmol/g wet tissue and (0.0155 +/- 0.0035) nmol/g wet tissue respectively, and the difference between them was very significant(P < 0.01).
CONCLUSIONSThe DHT-binding capacity of the DHT-binding protein in prostate is high and maintaining the high DHT level facilitates the effect of DHT.
