Progress in preparation of small monoclonal antibodies of knock out technique.
- Author:
Jing LIU
;
Xin-min MAO
;
Lin-lin LI
;
Xin-xia LI
;
Ye WANG
;
Yi LAN
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Monoclonal;
chemistry;
genetics;
immunology;
Humans;
Hybridomas;
metabolism;
Immunologic Techniques;
methods;
trends
- From:
China Journal of Chinese Materia Medica
2015;40(19):3737-3741
- CountryChina
- Language:Chinese
-
Abstract:
With the application of monoclonal antibody technology more and more widely, its production technology is becoming more and more perfect. Small molecule monoclonal antibody technology is becoming a hot research topic for people. The application of traditional Chinese medicine small molecule monoclonal antibody technology has been more and more widely, the technology for effective Chinese medicine component knockout provide strong technical support. The preparation of monoclonal antibodies and small molecule knockout technology are reviewed in this paper. The preparation of several steps, such as: in the process of preparation of antigen, hapten carrier coupling, coupling ratio determination and identification of artificial antigen and establishment of animal immunization and hybridoma cell lines of monoclonal antibody, the large-scale preparation; small molecule monoclonal antibody on Immune in affinity chromatography column method is discussed in detail. The author believes that this technology will make the traditional Chinese medicine research on a higher level, and improve the level of internationalization of Chinese medicine research.
