ITS sequences variation and phylogenetic analysis on 31 geographical populations of Notopterygium incisum.
- Author:
Lu-cun YANG
;
He-chun LIU
;
Xue-li ZHOU
;
Wen-hua XU
;
Guo-ying ZHOU
- Publication Type:Journal Article
- MeSH:
Apiaceae;
classification;
genetics;
Base Sequence;
China;
DNA, Intergenic;
genetics;
Gene Flow;
Genetic Variation;
Molecular Sequence Data;
Phylogeny
- From:
China Journal of Chinese Materia Medica
2015;40(19):3748-3753
- CountryChina
- Language:Chinese
-
Abstract:
In this study, 31 Notopterygium incisum populations were analyzed using ITS sequences to investigate the genetic structure. The results showed that: the ITS region ranged in size from 634 to 635 bp and base composition was with high G + C content of 57.8%. Thirty-one polymorphic sites were detected from 402 sequences of 31 populations of N. incisum, and the proportion of polymorphic sites was 4.88%, in which parsimony informative sites were up to 12. And 31 haplotypes were identified based on these polymorphic sites. Molecular variance analysis (AMOVA) indicated that high genetic differentiation (57%) existed among population, and gene flow was low (N(m) = 0.38) among populations. Phylogenetic relationships of 31 haplotypes were analyzed using NJ method with N. forbesiias an out-group. Phylogenetic analysis showed that 31 haplotypes from different populations mixed together and did not form distinct geographically separated clades.
