Optimization on alcohol precipitation techniques of Liuwei Dihuang decoction by response surface methodology.
- Author:
Ming YAN
;
Ying-chun WEI
;
Xue-feng LI
;
Jin MENG
;
Yun WU
;
Wei XIAO
- Publication Type:Journal Article
- MeSH:
Chemical Precipitation;
Chemistry, Pharmaceutical;
methods;
Drugs, Chinese Herbal;
chemistry;
Ethanol;
chemistry
- From:
China Journal of Chinese Materia Medica
2015;40(19):3794-3799
- CountryChina
- Language:Chinese
-
Abstract:
The theoretical basis of the alcohol precipitation process control was provided, the alcohol precipitation was optimized and the relationship equation was got. The monod glycoside, loganin and paeoniflorin were used as the evaluation indexes to determine the impact factors of alcohol precipitation techniques of Liuwei Dihuang decoction by the Plackett-Burman experimental design and the levels of non-significant factors were identified. Then, Box-Behnken response surface methodology was used to research and discuss the critical process parameters influence the effect of alcohol precipitation and draw interaction between key process parameters and the correlation equation with index components. Through the establishment and solving the quadratic regression model of composite score, the optimum preparation conditions of alcohol precipitation techniques of liuwei were as follows: stirring speed was 580 r x min(-1), standing time was 17 hours, alcohol concentration was 34%, the density of Liuwei Dihuang decoction was 1.13. The response surface methodology for optimized alcohol precipitation techniques of Liuwei Dihuang decoction is. reasonable and feasible.
