Investigation of metabolites of Triptergium wilfordii on liver toxicity by LC-MS.
- Author:
Xiao-mei ZHAO
;
Xin-ying LIU
;
Chang XU
;
Tao YE
;
Cheng JIN
;
Kui-jun ZHAO
;
Zhi-jie MA
;
Xiao-he XIAO
- Publication Type:Journal Article
- MeSH:
Animals;
Celastraceae;
chemistry;
metabolism;
toxicity;
Chromatography, Liquid;
methods;
Drugs, Chinese Herbal;
metabolism;
toxicity;
Liver;
drug effects;
metabolism;
Male;
Mass Spectrometry;
methods;
Rats;
Rats, Sprague-Dawley
- From:
China Journal of Chinese Materia Medica
2015;40(19):3851-3858
- CountryChina
- Language:Chinese
-
Abstract:
In this paper, biomarkers of liver toxicity of Triptergium wilfordii based on metabolomics was screened, and mechanism of liver toxicity was explored to provide a reference for the clinical diagnosis for liver toxicity of Triptergium wilfordii. MS method was carried on the analysis to metabolic fingerprint spectrum between treatment group and control group. The potential biomarkers were compared and screened using the multivariate statistical methods. As well, metabolic pathway would be detailed description. Combined with PCA and OPLS-DA pattern recognition analysis, 20 metabolites were selected which showed large differences between model group and blank group (VIP > 1.0). Seven possible endogenous biomarkers were analyzed and identified. They were 6-phosphate glucosamine, lysophospholipid, tryptophan, guanidine acetic acid, 3-indole propionic acid, cortisone, and ubiquinone. The level changes of above metabolites indicated that the metabolism pathways of amino acid, glucose, phospholipid and hormone were disordered. It is speculated that liver damage of T. wilfordii may be associated with the abnormal energy metabolism in citric acid cycle, amino acid metabolism in urea cycle, and glucose metabolism. It will be helpful to further research liver toxicity ingredients of Triptergium wilfordii.
