Studies on genetic diversity of three Tibetan herbs.
- Author:
Liang-hong NI
;
Zhi-li ZHAO
;
Jin-rong WU
;
Bo XIONG
;
Jia-ni LU
;
Dorje GAAWE
- Publication Type:Journal Article
- MeSH:
Genetic Variation;
Gentiana;
classification;
genetics;
Phylogeny;
Plant Proteins;
genetics;
Plants, Medicinal;
classification;
genetics;
Scrophularia;
classification;
genetics;
Swertia;
classification;
genetics;
Tibet
- From:
China Journal of Chinese Materia Medica
2015;40(19):3883-3888
- CountryChina
- Language:Chinese
-
Abstract:
The genetic diversity of three Tibetan herbs, i. e., Sang-Di, E-Dewa and Ye-Xingba (Tibetan names), was studied based on the field collection, specimen identification and DNA sequence analysis. Swertia hispidicalyx, Gentiana lhassica and Scrophularia dentata, as the original plants of the three Tibetan herbs, were collected and identified. The regions of ITS, matK, rbcL, rpoC1, trnL(UAA), psbA-trnH, atpB-rbcL, trnS (GCU)-trnG(UCC), rpl20-rps12, trnL(UAA)-trnF(GAA) and nadl 2nd intron were amplified and sequenced. The ITS regions of S. hispidicalyx and S. dentata were cloned and sequenced, and the sequences were classified into different genotypes. All the sequences were analyzed and compared with those of closely related species. Our studies may provide reference for the genetic diversity analysis and molecular identification of the three Tibetan herbs.
