Methylation status of zo-1 gene promoter in acute leukemia.
- Author:
Xin-Rong WANG
1
;
Xiao-Ning GAO
;
Hui-Yuan KANG
;
Li-Li WANG
;
Yong-Hui LI
;
Li YU
Author Information
1. Department of Hematology, Chinese PLA General Hospital, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Acute Disease;
Cell Line, Tumor;
DNA Methylation;
Humans;
Leukemia;
genetics;
Membrane Proteins;
genetics;
Phosphoproteins;
genetics;
Promoter Regions, Genetic;
Zonula Occludens-1 Protein
- From:
Journal of Experimental Hematology
2010;18(4):863-865
- CountryChina
- Language:Chinese
-
Abstract:
This study was purposed to investigate the difference of zo-1 gene promoter methylation between healthy individuals and acute leukemia patients. BS-PCR method was used to detect the status of zo-1 gene methylation in healthy individuals, acute leukemia patients and leukemic cell line NB4 cells. The results showed that zo-1 gene was hypomethylated in bone marrow samples from healthy individuals (1.9%). In newly diagnosed AL and relapsed patients, the rate of zo-1 gene methylation was 93.2% and 66.9% respectively, while it was 16.4% in AL patients in complete remission, which was much higher than that in healthy individuals. There was significant difference between them. It is concluded that as compared with healthy individuals, zo-1 gene in acute leukemia patients is hypermethylated and with different degrees in various phases of leukemia. Analysis of zo-1 gene methylation status may be useful to monitor the development of acute leukemia.
